anti wnt2  (Proteintech)

Journal: Pharmacology Research & Perspectives

Article Title: Lobaric Acid Exhibits Anticancer Potential by Modulating the Wnt/β‐Catenin Signaling Pathway in MCF‐7 Cells

doi: 10.1002/prp2.70142

Figure Lengend Snippet: Effect of LA on Wnt/β‐catenin pathway in MCF‐7 cells. (A) The migration of cells treated with LA into the wound was visualized at 0, 6, 12, and 24 h under an inverted microscope, and the percentage of wound closure was evaluated statistically. (B–I) Expressions of WNT2 , DVL1 , AXIN1 , β‐Catenin , TCF‐4 , CCND1 , c‐MYC , and CDK1 genes analyzed by qPCR in LA‐treated MCF‐7 cells at 48 h. (J–M) Protein expressions of WNT2, GSK3‐ β, and β‐catenin determined by Western blot analysis. The experiment was performed in three biological and technical replicates. * p < 0.05, ** p < 0.01, and *** p < 0.001 in relation to the control. Scale bar, 500 μm.

Article Snippet: Subsequently, membrane incubation was performed overnight at 4°C using P53 antibody (Proteintech, 10442‐1‐AP, 1:1000), BCL2 antibody (Proteintech 12789‐1‐AP 1:500), WNT2 antibody (Proteintech, 66656‐I‐Ig, 1:1000), AXIN antibody (Proteintech, 68093‐1‐Ig, 1:1000), β‐Catenin antibody (Santa Cruz Biotechnology, sc‐7963, 1:1000), GSK3‐ β antibody (Santa Cruz, sc‐377213, 1:1000), and β‐Actin antibody (Santa Cruz Biotechnology, sc‐47778, 1:1000).

Techniques: Migration, Inverted Microscopy, Western Blot, Control

Journal: Molecular Vision

Article Title: Cassia polysaccharides can regulate the effect of low PAX6 expression on the function of ARPE-19 cells through the Wnt/β-catenin pathway

doi:

Figure Lengend Snippet: Effect of PAX6-shRNA mediated by cassia polysaccharide on the Wnt/β-catenin pathway. A : After PAX6 gene knockdown, the mRNA expression of Wnt2 in the PAX6-shRNA group and XAV-939 group was significantly up-regulated compared with the normal group and cassia polysaccharide group. B : After treatment with the Wnt/β-catenin pathway inhibitor, the mRNA expression of β-catenin in the XAV-939 group was significantly decreased, while the mRNA expression trend of β-catenin in the cassia polysaccharide group showed no obvious change. C – E : After PAX6 gene knockdown, the protein expression trends of Wnt2 and β-catenin in the PAX6-shRNA group, XAV-939 group and cassia polysaccharide group were consistent with their respective mRNA expression trends, using the protein expression levels of the blank group and negative control group as references (n=3; *p<0.05, **p<0.01, ***p<0.001, respectively).

Article Snippet: Wnt2 antibody, β-catenin antibody, COL1A1 antibody, MMP-2 antibody, TGF-β antibody, cyclin D1 antibody, and proliferating cell nuclear antigen (PCNA) antibody were purchased from WanleiBio (Liaoning, China). β-Actin antibody and goat antimouse antibody were purchased from Beyotime Biotechnology (Shanghai, China).

Techniques: shRNA, Knockdown, Expressing, Negative Control